Résumés
Abstract
Root-knot nematode is a major constraint to tomato cultivation in open and protected structures. Resistance sources need to be continuously identified for combating pathogens affecting the yield. In the present studies, forty-seven genotypes of tomato were evaluated phenotypically along with their genotypic characterization. On the basis of their phenotypic reaction, the genotypes were grouped into four categories viz.: resistant, moderately resistant, susceptible and highly susceptible. Of these genotypes, only five were found to be resistant while forty-two were rated from moderately resistant to highly susceptible. Multiplication of Meloidogyne incognita was greatly reduced (Rf < 1) in resistant genotypes as compared to susceptible genotypes. Root galling index was also very low in resistant genotypes. Using molecular markers, the presence of the Mi-1.2 resistance gene was also confirmed in five resistant genotypes (L-0272, NR-14, L-097, L-0275 and PNR-7). These resistant sources could become a source of germplasm in breeding programs for the development of resistant cultivars.
Keywords:
- tomato,
- screening,
- Mi gene,
- resistance,
- Meloidogyne incognita
Résumé
Le nématode à galles est une contrainte majeure à la culture de la tomate dans des structures ouvertes et protégées. Les sources de résistance doivent être identifiées en permanence pour lutter contre les agents pathogènes affectant le rendement. Jusqu’à présent, quarante-sept génotypes de tomate ont été évalués phénotypiquement, de même que leur caractérisation génotypique. Selon leur réaction phénotypique, les génotypes ont été regroupés en quatre catégories : résistant, modérément résistant, sensible et très sensible. Parmi ces génotypes, seuls cinq se sont révélés résistants tandis que quarante-deux ont été classés de modérément résistants à très sensibles. La multiplication de Meloidogyne incognita était fortement réduite (Rf < 1) dans les génotypes résistants par rapport aux génotypes sensibles. L'indice de galles racinaires était également très faible dans les génotypes résistants. À l'aide de marqueurs moléculaires, la présence du gène de résistance Mi-1.2 a également été confirmée dans cinq génotypes résistants (L-0272, NR-14, L-097, L-0275 et PNR-7). Ces sources résistantes pourraient devenir une source de matériel génétique dans les programmes de sélection pour le dévelop-pement de cultivars résistants.
Mots-clés :
- tomate,
- criblage,
- gène Mi,
- résistance,
- Meloidogyne incognita
Parties annexes
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