Résumés
Résumé
Des antagonistes bactériens envers Helminthosporium solani ont été soumis à différents essais en vue de déterminer le rôle de l'antibiose dans l'antagonisme observé. Parmi les souches bactériennes évaluées, sept (Alcaligenes piechaudii, Aquaspirillum autotrophicum, Cellulomonas fimi, Pseudomonas chlororaphis, Pseudomonas putida (souches 94-19 et E-30) et Streptomyces griseus) ont produit dans le milieu gélosé des métabolites inhibant la croissance mycélienne et/ou la germination des conidies de H. solani. Les métabolites produits en culture pure et en culture mixte ont présenté une activité différente sur la croissance mycélienne de H. solani. Les résultats présentés indiquent que la méthodologie employée peut être un facteur déterminant dans l'identification de l'antibiose comme mode d'action d'un agent antagoniste.
Mots clés:
- Antagonistes bactériens,
- antibiose,
- Helminthosporium solani,
- métabolites,
- gale argentée de la pomme de terre
Abstract
Bacterial antagonists of Helminthosporium solani were submitted to different tests in order to determine the role of antibiosis in their antagonistic interaction. Among the bacterial strains tested, seven (Alcaligenes piechaudii, Aquaspirillum autotrophicum, Cellulomonas fimi, Pseudomonas chlororaphis, Pseudomonas putida (strains 94-19 and E-30) and Streptomyces griseus) were shown to produce agar diffusible metabolites inhibiting H. solani mycelial growth and/or conidial germination. Differential activity was revealed when diffusible metabolites were extracted from either pure cultures of these antagonists or dual culture in the presence of H. solani. The results presented indicate that the methodology employed could be a decisive factor in whether or not antibiosis can be identified as a mode of action of biocontrol agents.
Keywords:
- Antibiosis,
- bacterial antagonists,
- diffusible metabolites,
- Helminthosporium solani,
- potato silver scurf
Parties annexes
References
- Assis, S.M.P., E.B. Silveira, R.L.R. Mariano, and D. Menezes. 1998. Endophytic bacteria - Method for isolation and antagonistic potential against cabbage black rot. Summa Phytopathol. 24 : 216-220.
- Avis, T.J., R.R. Boulanger, and R.R. Bélanger. 2000. Synthesis and biological characterization of (z)-9-heptadecenoic and (z)-6-methyl-9-heptadecenoic acids: fatty acids with antibiotic activity produced by Pseudozyma flocculosa. J. Chem. Ecol. 26 : 987-1000.
- Bélanger, R.R., N. Dufour, J. Caron, and N. Benhamou. 1995. Chronological events associated with the antagonistic properties of Trichoderma harzianum against Botrytis cinerea: indirect evidence for sequential role of antibiosis and parasitism. Biocontrol Sci. Technol. 5 : 41-54.
- Chin-A-Woeng, T.F.C., G.V. Bloemberg, A.J. van der Bij, K.M.G.M. van der Drift, J. Schripsema, B. Kroon, R.J. Scheffer, C. Keel, P.A.H.M. Bakker, H.V. Tichy, F.J. de Bruijn, J.E. Thomas-Oates, and B.J.J. Lugtenberg. 1998. Biocontrol by phenazine-1-carboxamide-producing Pseudomonas chlororaphis PCL1391 of tomato root rot caused by Fusarium oxysporum f. sp. radicis-lycopersici. Mol. Plant-Microbe Interact. 11 : 1069-1077.
- Errampalli, D., J.M. Saunders, and J.D. Holley. 2001. Emergence of silver scurf (Helminthosporium solani) as an economically important disease of potato. Plant Pathol. 50 : 141-153.
- Fravel, D.R. 1988. Role of antibiosis in the biocontrol of plant diseases. Annu. Rev. Phytopathol. 26 : 75-91.
- Handelsman, J., S. Raffel, E.J. Mester, L. Wunderlich, and C.R. Gau. 1990. Biological control of damping-off of alfalfa seedlings with Bacillus cereus UW85. Appl. Environ. Microbiol. 56 : 713-718.
- Handelsman, J., W.C. Nesmith, and S.J. Raffel. 1991. Microassay for biological and chemical control of infection of tobacco by Phytophthora parasitica var. nicotianae. Curr. Microbiol. 22 : 317-319.
- Hiroyuki, C., A. Hitosi, K. Rei, T. Tadashi, S. Kasuya, D. Kazuyuki, and Y. Takeo. 1999. Rhodopeptins (Mer-N1033), novel cyclic tetrapeptides with antifungal activity from Rhodococcus sp.: I. Taxonomy, fermentation, isolation, physico-chemical properties and biological activities. J. Antibiot. 52 : 695-699.
- Howell, C.R. and R.D. Stipanovic. 1979. Control of Rhizoctonia solani on cotton seedlings with Pseudomonas fluorescens and with an antibiotic produced by the bacterium. Phytopathology 69 : 480-482.
- Huddleston, A.S., N. Cresswell, M.C.P. Neves, J.E. Beringer, S. Baumberg, D.I. Thomas, and E.M.H. Wellington. 1997. Molecular detection of streptomycin-producing Streptomycetes in Brazilian soils. Appl. Environ. Microbiol. 63 : 1288-1297.
- Jung, H.K., and S.D. Kim. 2004. Selection and antagonistic mechanism of Pseudomonas fluorescens 4059 against phythophthora blight disease. Korean J. Microbiol. Biotechnol. 32 : 312-316.
- Lynch, J.M. 1990. Microbial metabolites. Pages 177-206 in J.M. Lynch (ed.), The Rhizosphere. John Wiley & Sons, New York.
- Marchand, P.A., D.M. Weller, and R.F. Bonsall. 2000. Convenient synthesis of 2,4-diacetylphloroglucinol, a natural antibiotic involved in the control of take-all disease of wheat. J. Agric. Food Chem. 48 : 1882-1887.
- Martinez, C., M. Michaud, R.R. Bélanger, and R.J. Tweddell. 2002. Identification of soils suppressive against Helminthosporium solani, the causal agent of potato silver scurf. Soil Biol. Biochem. 34 : 1861-1868.
- Mérida, C.L., and R. Loria. 1994. Comparison of thiabendazole-sensitive and-resistant Helminthosporium solani isolates from New York. Plant Dis. 78 : 187-192.
- Michaud, M., C. Martinez, A.-M. Simao-Beaunoir, R.R. Bélanger, and R.J. Tweddell. 2002. Selection of antagonist microorganisms against Helminthosporium solani, causal agent of potato silver scurf. Plant Dis. 86 : 717-720.
- Molet, B., J. Peel, D. Pridmore, N. Rekhif, and B. Suri. 2004. Bactericide compositions prepared and obtained from Micrococcus varians. Off. Gaz. U.S. Pat. Trademark Off. Pat. p. 1279.
- O’Mahony, T., N. Rekhit, C. Cavadini, and G.F. Fitzgerald. 2001. The application of a fermented food ingredient containing ‘variacin’ a novel antimicrobial produced by Kocuria varians, to control the growth of Bacillus cereus in chilled dairy products. J. Appl. Microbiol. 90 : 106-114.
- Ongena, M., F. Duby, F. Rossignol, M.L. Fauconnier, J. Dommes, and P. Thonart. 2004. Stimulation of the lipoxygenase pathway is associated with systemic resistance induced in bean by a nonpathogenic Pseudomonas strain. Mol. Plant-Microbe Interact. 17 : 1009-1018.
- Papou, F.A., and N.M. Gryn`ko. 1994. Ecologically safe methods of protection of seed white head cabbage and winter cauliflower. Vesti Akademii Agrarnykh Navuk Belarussi 3 : 54-59.
- Platt, H.W. 1997. Resistance to thiabendazole in Fusarium species and Helminthosporium solani in potato tubers treated commercially in eastern Canada. Phytoprotection 78 : 1-10.
- Secor, G.A., and N. Gudmestad. 1999. Managing fungal diseases of potato. Can. J. Plant Pathol. 21 : 213-221.
- Séveno, N.A., J.A.W. Morgan, and E.M.H. Wellington. 2001. Growth of Pseudomonas aureofaciens PGS12 and the dynamics of HHL and phenazines production in liquid culture, on nutrient agar, and on plant roots. Microb. Ecol. 41 : 314-324.
- Silo-Suh, L.A., B.J. Lethbridge, S.J. Raffel, H. He, J. Clardy, and J. Handelsman. 1994. Biological activities of two fungistatic antibiotics produced by Bacillus cereus UW85. Appl. Environ. Microbiol. 60 : 2023-2030.
- Smith, K.P., M.J. Havey, and J. Handelsman. 1993. Suppression of cottony leak of cucumber with Bacillus cereus strain UW85. Plant Dis. 77 : 139-142.
- Sonnenbichler, J., J. Dietrich, and H. Peipp. 1994. Secondary fungal metabolites and their biological activities. V. Investigations concerning the induction of the biosynthesis of toxic secondary metabolites in basidiomycetes. Biol. Chem. Hoppe-Seyler 375 : 71-79.
- Sonnenbichler, J., J.J. Guillaumin, H. Peipp, and D. Schwarz. 1997. Secondary metabolites from dual cultures of genetically different Armillaria isolates. Eur. J. For. Pathol. 27 : 241-249.
- Sultana, R., B.S. Siddiqui, K. Taraz, H. Budzikiewicz, and J.M. Meyer. 2001. An isopyoverdin from Pseudomonas putida CFML 90-33. Tetrahedron 57 : 1019-1023.
- Thrane, C., T.H. Nielsen, M.N. Nielsen, J. Sorensen, and S. Olsson. 2000. Viscosinamide-producing Pseudomonas fluorescens DR54 exerts a biocontrol effect on Pythium ultimum in sugar beet rhizosphere. FEMS Microbiol. Ecol. 33 : 139-146.
- Whipps, J.M. 1987. Effect of media on growth and interactions between a range of soil-borne glasshouse pathogens and antagonistic fungi. New Phytol. 107 : 127-142.
10.7202/706113ar