Abstracts
Résumé
Le présent travail reporte l’effet du cadmium (Cd), du benzo[a]pyrène (B[a]P) ainsi que leur mélange (Cd/B[a]P), à 1 µM, sur les activités d’enzymes impliqués dans la phase I et la phase II de biotransformation chez le polychète Nereis diversicolor en fonction du temps (après 12, 24, 36 et 48 h).
L’effet d’une contamination aiguë par du cadmium à une dose de 1 µM après 12, 24 et 36 h montre une inhibition de l’activité NADPH cytochrome C réductase chez les individus contaminés comparés à leurs témoins relatifs, alors que le seul effet du cadmium sur l’activité glutathion-S-transférase n’est enregistré qu’après 36 h d’exposition. Quant au benzo[a]pyrène, les résultats montrent une augmentation significative de l’activité NADPH cytochrome C réductase après 12, 24 et 36 h d’exposition, alors que pour l’activité glutathion-S‑transférase, la variation significative entre les animaux témoins et traités n’est enregistrée qu’à 36 h d’exposition. Le mélange (Cd/B[a]P) inhibe l’activité NADPH cytochrome C réductase chez les individus traités par comparaison aux témoins relatifs et montre un effet inducteur sur l’activité GST sauf après 36 h d’exposition. Ces résultats montrent ainsi les interactions entre les polluants ainsi que leurs effets sur les organismes.
Mots clés:
- Nereis diversicolor,
- cadmium,
- benzo[a]pyrène,
- NADPH cytochrome C reductase et glutathion-S-transferase
Abstract
The acute effects of cadmium and benzo[a]pyrene, individually and as a mixture, were evaluated on the phase I and phase II biotransformation enzymes of Nereis diversicolor (Polychaeta) collected from unpolluted sites. In the acute assay (1 µM) after 12, 24, 36 and 48 h, the comparison between worms exposed to cadmium and their controls showed an inhibited activity of NADPH cytochrome C reductase after 12, 24 and 36 h. For the activity of glutathione-S-transferase (GST), however, inhibition in the presence of Cd was only noted after 36 h of exposure. Although exposure to benzo[a]pyrene resulted in a significant increase in NADPH cytochrome C reductase activity after 12, 24 and 36 h, a significant difference in glutathione-S-transferase (GST) activity between treated and control animals was only observed after 36 h. The mixture (Cd/B[a]P) seemed to inhibit the activity of NADPH cytochrome C reductase and to induce the activity of GST in individuals but only up until 36 h of exposure. These findings illustrate the interactions between pollutants and their effects on organisms.
Keywords:
- Nereis diversicolor,
- cadmium,
- benzo[a]pyrene,
- NADPH cytochrome C reductase and glutathione-S-transferase
Appendices
Références bibliographiques
- AKCHA F., C. IZUEL, P. VENIER, H. BUDZINSKI, T. BURGEOT, et J.F. NARBONNE (2000). Enzymatic biomarker measurement and study of DNA adduct formation in benzo(a)pyrene contaminated mussels Mytilus galloprovincialis.Aquat Toxicol., 49, 269-287.10.1016/S0166-445X(99)00082-X Google Scholar Search this bibliographic reference on Google Scholar
- AMIARD J.C., A. GEFFARD, C. AMIARD-TRIQUET et C. CROUZET (2007). Relationship between the lability of sediment-bound metals (Cd, Cu, Zn) and their bioaccumulation in benthic invertebrates. Estuar. Coast. Shelf Sci., 72, 511-521.10.1016/j.ecss.2006.11.017 Google Scholar Search this bibliographic reference on Google Scholar
- BANNI M. (2004). Étude biochimique, moléculaire et toxicologique de l’effet des contaminants sur l’environnement marin : cas du littoral tunisien. Thèse de doctorat, Univ. Monastir, Tunisie,144 p.10.1080/13547500500107497 Google Scholar Search this bibliographic reference on Google Scholar
- BANNI M., J. JEBALI, M. DAUBEZE, C. CLERANDEAU, H. GUERBEJ, J.F. NARBONNE et H. BOUSSETTA (2005). Monitoring pollution in Tunisian coasts: application of a classification scale based on biochemical markers. Biomarkers, 10, 105-116.10.1080/13547500500107497 Google Scholar Search this bibliographic reference on Google Scholar
- BAROUKI R. et Y. MOREL (2001). Repression of cytochrome P450 1A1 gene expression by oxidative stress: mechanisms and biological implications. Biochem. Pharmacol., 61, 511‑516.10.1016/S0006-2952(00)00543-8 Google Scholar Search this bibliographic reference on Google Scholar
- BENEDETTI M., G. MARTUCCIO, D. FATTORINI, A. CANAPA, M. BARUCCA, M. NIGRO et F. REGOLI (2007). Oxidative and modulatory effects of trace metals on metabolism of polycyclic aromatic hydrocarbons in the Antarctic fish Trematomus bernacchii. Aquat Toxicol., 85,167-175.10.1016/j.aquatox.2007.08.009 Google Scholar Search this bibliographic reference on Google Scholar
- BJERREGAARD P. et M.H. DEPLEDGE (1994). Cadmium accumulation in Littorina littorea, Mytilus edulis and Carcinus maenas: the influence of salinity and calcium ion concentrations. Mar. Biol., 119, 385-395.10.1007/BF00347535 Google Scholar Search this bibliographic reference on Google Scholar
- BOURAOUI Z, M. BANNI, J. GHEDIRA, C. CLERANDEAU, H. GUERBEJ, J.F. NARBONNE et H. BOUSSETTA (2008). Acute effects of cadmium on liver phase I and phase II enzymes and metallothionein accumulation on sea bream Sparus aurata. Fish Physiol. Biochem., 34, 201-207.10.1007/s10695-007-9177-y Google Scholar Search this bibliographic reference on Google Scholar
- BRADFORD M.M. (1976). A rapid and sensitive method for the quantification of microgram of protein utilizing the principal of protein-dye binding. Anal Biochem., 72, 248‑254.10.1016/0003-2697(76)90527-3 Google Scholar Search this bibliographic reference on Google Scholar
- CARPENTER D.O., K. ARCARO, D.C. SPINK (2002). Understanding the human health effects of chemical mixtures. Environ. Health Perspect., 110, 25-42.10.1289/ehp.02110s125 Google Scholar Search this bibliographic reference on Google Scholar
- ERKUDEN P., B. JULIAN et S. MONTSERRAT (2004). Biomarker responses to pollution in tow invertebrate species: Scrobicularia plana and Nereis diversicolor from the Cadiz Bay (SW Spain). Mar. Environ. Res., 58,275-279.10.1016/j.marenvres.2004.03.071 Google Scholar Search this bibliographic reference on Google Scholar
- GOERING P.L., P. MISTRY et B.A. FOWLER (1987). Mechanisms of metal induced cell injury. Dans : Handbook of Toxicology, Haley T.J. et W.O. Berndt (Éditeurs), Hemisphere Publishing Corporation, New-York, Chap 10, pp. 384-425.10.1016/j.tox.2004.04.011 Google Scholar Search this bibliographic reference on Google Scholar
- GRAVATO, C. et M.A. SANTOS (2002). Juvenile sea bass liver P450, EROD induction and erythrocytic genotoxic responses to PAH and PAH-like compounds. Ecotox. Environ. Saf., 51, 115-127.10.1006/eesa.2001.2133 Google Scholar Search this bibliographic reference on Google Scholar
- GILLET P. (1993). Impact de l’implantation d’un barrage sur la dynamique des populations de Nereis diversicolor (Annelide Polychete) de l’estuaire Du Bou Regreg, Maroc. J. Rech. Océanogr., 18,15-18.10.1016/0166-445X(94)00073-Y Google Scholar Search this bibliographic reference on Google Scholar
- HABIG W.H., M.J. PABST et W.B. JAKOBY (1974). Glutathione S-transferases. The first enzymatic step in mercapturic acid formation. J. Biol. Chem., 249, 7130‑7139.10.1016/j.mrgentox.2007.04.015 Google Scholar Search this bibliographic reference on Google Scholar
- HAYES, A.W. (1982). Principles and methods of toxicology. Raven Press, New york, NY, USA, 612 p.10.1016/j.tox.2008.02.017 Google Scholar Search this bibliographic reference on Google Scholar
- KORASHY H.M. et A.O.S. EL-KADI (2004). Differential effects of mercury, lead and copper on the constitutive and inducible expression of aryl hydrocarbon receptor (AHR)-regulated genes in cultured hepatoma Hepa 1c1c7 cells. Toxicol., 201, 153-172.10.1016/j.tox.2004.04.011 Google Scholar Search this bibliographic reference on Google Scholar
- LEE B.G, S.B. GRISCOM, J.S. LEE, H.G. CHOI, C.H. KOH, S.N. LUONA et N.S. FICHER (2000). Influence of dietary uptake and reactive sulfides on metal bioavaibility from aquatic sediments. Science, 287, 282-284.10.1126/science.287.5451.282 Google Scholar Search this bibliographic reference on Google Scholar
- LEE R.F. et S.C. SINGER (1980). Detoxifying enzymes system in marine polychaetes; increases in activity after exposure to aromatic hydrocarbons. Rapp., p-v. réunion, Cons. Int. Explor. Mer, 179, 29-32.10.1007/978-1-4613-3318-0_6 Google Scholar Search this bibliographic reference on Google Scholar
- LEMAIRE-GONY S., P. LEMAIRE et A.L. PULSFORD (1995). Effects of cadmium and benzo(a)pyrene on the immune system, gill ATPase and EROD activity of European sea bass Dicentrarchus labrax. Aquat. Toxicol., 31, 297-313.10.1016/0166-445X(94)00073-Y Google Scholar Search this bibliographic reference on Google Scholar
- LEWINSKA D., J. ARKUSZ, M. STANCZYK, J. PALUS, E. DZIUBALTOWSKA et M. STEPNIK (2007). Comparison of the effects of arsenic and cadmium on benzo(a)pyrene-induced micronuclei in mouse bone-marrow. Mutat. Res., 632, 37-43.10.1016/j.mrgentox.2007.04.015 Google Scholar Search this bibliographic reference on Google Scholar
- LIN T., N.K. MAK, M.S. YANG (2008). MAPK regulate p53-dependent cell death induced by benzo[a]pyrene: Involvement of p53 phosphorylation and acetylation. Toxicol., 247, 145-153.10.1016/j.tox.2008.02.017 Google Scholar Search this bibliographic reference on Google Scholar
- LINDAMOOD C. (1991). Xenobiotic biotransformation. Dans : Hepatotoxicology. MEEKS R.G., S.D. HARRISON et R.J. BULL (Éditeurs). C.R.C Press, 1, pp. 139-180.10.1016/S0166-445X(96)00838-7 Google Scholar Search this bibliographic reference on Google Scholar
- LIVINGSTONE D.R, P. LEMAIRE, A. MATTEWS, L. PETERS, D. BUCKE et W.R. AMLAP (1993). Pro-oxidant, anti-oxidant and 7-Ethoxyresorufin-O-deethylase (EROD) activity responses in liver of Dab (Limanda limanda) exposed to sediment contaminated with hydrocarbons and other chemicals. Mar. Pollut. Bull., 26, 602-606.10.1016/0025-326X(93)90498-9 Google Scholar Search this bibliographic reference on Google Scholar
- METCALFE C.D. (1988). Induction of micronuclei and nuclear abnormalities in the erythrocytes of mudminnows (Umbra limi) and brown bullhead (Ictalurus nebulosus). Bull. Environ. Contam. Toxicol., 40, 489-495.10.1007/BF01688371 Google Scholar Search this bibliographic reference on Google Scholar
- METTAM C. (1981). Survival strategies in estuarine Nereids. Dans : Feeding and Survival Strategies of Estuarine Organisms. JONES N.V. et W.J. WOLFF (Éditeurs), Macmillan Publishing Co., New-York, USA, pp. 65-77.10.1007/978-1-4613-3318-0_6 Google Scholar Search this bibliographic reference on Google Scholar
- OLIVEIRA M., M.A. SANTOS et M. PACHECO (2004). Glutathione protects heavy metal-induced inhibition of hepatic microsomal ethoxyresorufin-O-deethylase activity in Dicentrarchus labrax L.Ecotoxicol. Environ. Saf., 58, 379‑385.10.1016/j.ecoenv.2004.03.003 Google Scholar Search this bibliographic reference on Google Scholar
- PACHECO M. et M.A. SANTOS (1997). Induction of liver EROD activity and genotoxic effects by polycyclic aromatic hydrocarbons and resin acids on the juvenile eel (Anguilla anguilla). Ecotoxicol. Environ. Saf., 38, 252-259.10.1006/eesa.1997.1585 Google Scholar Search this bibliographic reference on Google Scholar
- PALMQVIST A., H. SELCK, L.J. RASMUSSEN et V.E. FORBES (2003). Biotransformation and genotoxicity of fluoranthene in the deposit-feeding polychaete Capitella sp. I. Environ. Toxicol. Chem., 22, 2977-2985.10.1897/02-474 Google Scholar Search this bibliographic reference on Google Scholar
- PETERS L.D, H.R. MORSE, R. WATERS et D.R. LIVINGSTONE (1997). Responses of hepatic cytochrome P4501A and formation of DNA adducts in juveniles of Turbot (Scophtalmus maximus L) exposed to water-borne benzo[a]pyrene. Aquat. Toxicol., 38, 67-82.10.1016/S0166-445X(96)00838-7 Google Scholar Search this bibliographic reference on Google Scholar
- RAINBOW P.S. (1997). Ecophysiology of trace metal uptake in crustaceans. Estuar. Coast. Shelf Sci., 44, 169-175.10.1006/ecss.1996.0208 Google Scholar Search this bibliographic reference on Google Scholar
- RISSO-DE FAVERNEY C., M. LAFAURIE, J.P. GIRARE et R. RAHMANI (2000). The nitroxide stable radical tempo prevents metal-induced inhibition of CYP1A1 expression and induction. Toxicol. Lett., 111, 219-227.10.1016/S0378-4274(99)00183-6 Google Scholar Search this bibliographic reference on Google Scholar
- REGOLI F., M. NIGRO, M. BENEDETTI, S. GORBI, C. PRETTI, P.G. GERVASI et D. FATTORINI (2005). Interactions between metabolism of trace metals and xenobiotics agonists of the Ah receptor in the Antarctic fish Trematomus bernacchii: environmental perspectives. Environ. Toxicol. Chem., 24, 1475-1482.10.1897/04-514R.1 Google Scholar Search this bibliographic reference on Google Scholar
- ROAST S.D., J. WIDDOWS et M.B JONES (2001). Effects of salinity and chemical speciation on cadmium accumulation and toxicity to two mysid species. Environ. Toxicol. Chem., 20, 1078-1084.Google Scholar Search this bibliographic reference on Google Scholar
- SANDRINI J.Z, J.V. LIMA, F. REGOLI, F. FATTORINI, A. NOTTI, L.F. MARINS et J.M. MONSERRAT (2008). Antioxidant responses in the nereidid Laeonereis acuta (Annelida, Polychaeta) after cadmium exposure. Ecotoxicol. Environ. Saf., 70, 115-120.10.1016/j.ecoenv.2007.03.004 Google Scholar Search this bibliographic reference on Google Scholar
- SORRENTINO C., N.K. ROY, S.C. COURTENAY et I. WIRGIN (2005). Co-exposure to metals modulates CYP1A mRNA inducibility in Atlantic tomcod Microgadus tomcod from two populations. Aquat. Toxicol., 75, 238-252.10.1016/j.aquatox.2005.08.006 Google Scholar Search this bibliographic reference on Google Scholar
- STEPHENSEN E., J. SVAVARSSON, J. STURVE, G. ERICON, M. ADOLFSON-ERICI et L. FORLIN (2000). Biochemical indicators of pollution exposure in shorthorn sculpin (Myoxocephalus scorpius), caught in four harbours on the south-west coast of Iceland. Aquat. Toxicol., 48, 431-442.10.1016/S0166-445X(99)00062-4 Google Scholar Search this bibliographic reference on Google Scholar
- SUN F.H et K.X. ZHOU (2008). Oxidative stress biomarkers of the polychaete Nereis diversicolor exposed to cadmium and petroleum hydrocarbons. Ecotoxicol. Environ. Saf., 70,106-114.10.1201/9780367812041-4 Google Scholar Search this bibliographic reference on Google Scholar
- VAN DEN HURK P., M. FAISAL et M.H. ROBERTS (2000). Interactive effects of cadmium and benzo[a]pyrene on metallothionein induction in mummichog (Fundulus heteroclitus). Mar. Environ. Res., 50, 83-87.10.1016/S0141-1136(00)00098-2 Google Scholar Search this bibliographic reference on Google Scholar
- VERBOST P.M., J. VAN ROOIJ, G. FLIK, R.A.C. LOCK et S.E. WENDELAAR BONGA (1989). The movement of cadmium through freshwater trout branchial epithelium and its interference with calcium transport. J. Exp. Biol., 145, 185-197.10.1016/j.ecoenv.2007.04.014 Google Scholar Search this bibliographic reference on Google Scholar
- VIARENGO A., L. CANESI, M. PERTICA, G. POLI, M.N. MOORE et M. ORUNESU (1990). Heavy metal effects on lipid peroxidation in the tissues of Mytilus galloprovincialisLam. Comp. Biochem. Physiol., 97C, 37-42.10.1242/jeb.145.1.185 Google Scholar Search this bibliographic reference on Google Scholar
- VIERA L.R., A. SOUSA, M.F. FRASCOA, I. LIMA, F. Morgado et L. GUILHERMINO (2008). Acute effects of Benzo[a]pyrene, anthracene and a fuel oil on biomarkers of the common goby Pomatoschistus microps (Teleostei, Gobiidae). Sci. Total Environ., 395, 87-100.10.1016/0742-8413(90)90168-9 Google Scholar Search this bibliographic reference on Google Scholar
- WAISBERG M., P. JOSEPH, B. HALE et D. BAYERSMANN (2003). Molecular and cellular mechanisms of cadmium carcinogenesis. Toxicol., 192, 95-117.10.1016/S0300-483X(03)00305-6 Google Scholar Search this bibliographic reference on Google Scholar