Abstracts
Abstract
In an attempt to cause fatal infection of wireworm Agriotes obscurus [Coleoptera: Elateridae], Metarhizium anisopliae was applied in the field as factorial combinations of conidia formulated as granules at 3.68 g granules or 1.25 x 1010 cfu per 196 cm2 (6.38 x 107 conidia cm-2), as conidia mixed with soil at 1.26 x 1010 cfu per 2.986 cm3 soil (4.22 x 106 conidia cm-3 soil), and as conidia-coated wheat seed (100 wheat seeds or 4.16 x 109 cfu per 196 cm2 = 2.12 x 107 conidia cm-2). The treatments resulted in a significantly greater number of mycosed wireworms compared with the control over and during five sampling periods. Significant differences in total wireworm mortality and mycosed wireworms in the field occurred at any time ranging from 15 to 82 d following treatment. The treatments also caused a reduction in the number of wireworms found in the cores, implying that they had a repellent effect. Latent infection of wireworms became apparent after living wireworms from the field treatments died following incubation under laboratory conditions, in numbers significantly greater than the control. This study showed that M. anisopliae can be applied in the field and infect and kill wireworms, but only at concentrations exceeding 4 x 106 conidia cm-3 with the subject isolate, wireworm species, and field conditions used in this study.
Keywords:
- Agriotes,
- biocontrol,
- biopesticide,
- Elaterididae,
- Metarhizium,
- microbial control,
- mycoinsecticide,
- wireworm
Résumé
Dans une tentative d'infecter mortellement le ver fil de fer Agriotes obscurus [Coleoptera: Elateridae], le Metarhizium anisopliae a été appliqué au champ selon des combinaisons factorielles d'une formulation granulaire de conidies à 3,68 g de granules ou 1,25 x 1010 ufc par 196 cm2 (6,38 x 107 conidies cm-2), de conidies mélangées à du sol à 1,26 x 1010 ufc par 2,986 cm3 de sol (4,22 x 106 conidies cm-3 de sol) et de graines de blé enrobées de conidies (100 graines de blé ou 4,16 x 109 ufc par 196 cm2 = 2,12 x 107 conidies cm-2). Pendant cinq périodes d'échantillonnage, un nombre significativement plus grand de vers fil de fer mycosés a été observé pour les traitements comparativement au témoin. Des différences significatives en termes de mortalité totale des vers fil de fer et de vers fil de fer mycosés au champ sont apparues à toutes les périodes, variant de 15 à 82 j après le traitement. Les traitements ont aussi réduit le nombre de vers fil de fer trouvés dans le cylindre central, ce qui indique qu'ils ont eu un effet répulsif. L'infection latente des vers fil de fer s'est manifestée lorsque des spécimens vivants provenant des traitements au champ sont morts après incubation en laboratoire, en nombres significativement plus élevés que ceux provenant du témoin. Les résultats démontrent que le M. anisopliae peut être utilisé au champ et peut infecter et tuer les vers fil de fer, mais seulement à des concentrations excédant 4 x 106 conidies cm-3 en utilisant l'isolat, le ver fil de fer, ainsi que les conditions décrites dans la présente étude.
Mots clés:
- Agriotes,
- biopesticide,
- Elaterididae,
- lutte biologique,
- lutte microbienne,
- Metarhizium,
- mycoinsecticide,
- ver fil de fer
Appendices
References
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