Abstracts
Résumé
L’imagerie cellulaire et tissulaire connaît un développement considérable résultant à la fois de l’attente des scientifiques et des progrès concertés de nombreuses disciplines. Le développement de la microscopie confocale, de la vidéomicroscopie, de nouveaux fluorochromes, notamment des dérivés de la GFP (green fluorescent protein), et de nouveaux logiciels permettent d’accéder à la visualisation tridimensionnelle des processus biologiques. Leur quantification à l’échelle de la cellule unique est maintenant possible. L’imagerie cellulaire fournit des résultats pertinents sur la stoechiométrie et la dynamique des interactions moléculaires impliquées dans les régulations cellulaires. Des progrès importants ont également été réalisés dans l’optimisation de la résolution temporelle. À l’époque de la post-génomique, une utilisation rationnelle des approches d’imagerie passe par une bonne connaissance de leurs avantages et de leurs limites.
Summary
Cell and tissue imaging provides scientists with wonderful tools, thanks to a fruitful dialog between chemistry, optical, mechanical, computational sciences and biology. Confocal microscopy, videomicroscopy together with a new generation of fluorochromes (especially those derived from green fluorescent protein, GFP) and image analysis software allow to visualize life in all its dimensions (space and time). Cell imaging also allows to quantify biological processes at the cellular level, to analyse both stoechiometry and dynamics of molecular interactions involved in cell and tissue regulations. Entering the new era of post-genomics requires a better knowledge of advantages and limitations of these new approaches.
Appendices
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