Abstracts
Abstract
Test tube plantlets were used to assess the transmission efficiency of Potato virus Y (PVY) from and to potato plants by the potato colonizing species green peach aphid (Myzus persicae) and the non-potato colonizing species soybean aphid (Aphis glycines). Similar levels of transmission of PVY by M. persicae were observed in the test tube plantlets and potted plants, demonstrating the reliability of this test for PVY transmission efficiency assay for aphids. The assay was then used to assess the transmission of PVYO and PVYN:O by M. persicae and A. glycines with two virus acquisition regimes, one with 5-min continuous probing and the other with 1-h acquisition access. The M. persicae mediated-transmission rate was 24.1% and 51.7% for PVYO and PVYN:O, respectively, under the 5-min acquisition regime; under the same acquisition regime, A. glycines led to 0.0% and 1.7% infection rates for PVYO and PVYN:O, respectively. Under the 1-h acquisition regime, no infection was observed except for PVYN:O by M. persicae, which exhibited an infection rate of 3.4%.
Keywords:
- Aphis glycines,
- Myzus persicae,
- plantlets,
- potato,
- Potato virus Y,
- test tube,
- transmission
Résumé
Des vitroplants ont été utilisés pour évaluer le taux de transmission du virus Y de la pomme de terre (PVY) entre plants de pomme de terre par un puceron inféodé à la pomme de terre, le puceron vert du pêcher (Myzus persicae), et un puceron non-inféodé à la pomme de terre, le puceron du soya (Aphis glycines). Des taux similaires d’efficacité de transmission du PVY par M. persicae ont été observés lorsque des vitroplants ou des plantes en pot étaient utilisés, ce qui démontre la validité de l’utilisation de vitroplants pour l’évaluation du taux de transmission du PVY par les pucerons. La même approche a par la suite été utilisée pour mesurer le taux de transmission du PVYO et du PVYN:O par M. persicae et par A. glycines sous deux régimes d’acquisition, soit 5 min de sondage continu et 1 h d’accès. Le taux de transmission de M. persicae a été de 24,1 % et de 51,7 % pour PVYO et PVYN:O, respectivement, sous un régime d’acquisition de 5 min, et de 0,0 % et 1,7 % pour PVYO et PVYN:O, respectivement, avec A. glycines. Sous un régime d’acquisition d’une heure, aucune infection n’a été observée à l’exception de PVYN:O par M. persicae qui a présenté un taux d’infection de 3,4 %.
Mots clés:
- Aphis glycines,
- Myzus persicae,
- vitroplants,
- virus Y de la pomme de terre,
- transmission
Appendices
References
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