Abstracts
Résumé
Bien que des progrès considérables aient été réalisés ces dernières années dans la compréhension des processus tumoraux, les retombées cliniques pour le diagnostic précoce des tumeurs demeurent limitées. L’avènement récent de nouveaux outils de protéomique, faisant appel à des techniques de biopuces à protéines, a totalement modifié les perspectives dans ce domaine et permis l’émergence du concept de profil d’expression protéique, véritable signature moléculaire de la tumeur. Sur la base de ce profil, la détection des cancers pendant la phase asymptomatique pourrait constituer une réelle avancée pour le patient et sa prise en charge thérapeutique. Cet article présente la plate-forme protéomique SELDI-TOF et ses premières applications en cancérologie. Toutefois, si ces applications laissent présager de multiples développements en cancérologie clinique, de nombreuses améliorations restent à faire avant leur implantation en routine hospitalière.
Summary
A key challenge in clinical proteomic of cancer is the identification of biomarkers that would allow early detection, diagnosis and monitor progression of the disease to improve long-term survival of patients. Recent advances in proteomic instrumentation and computational methodologies offer unique chance to rapidly identify these new candidate markers or pattern of markers. The combination of retentate affinity chromatography and surfaced-enhanced laser desorption/ionization time-of-flight (SELDI-TOF) mass spectrometry is one of the most interesting new approaches for cancer diagnostic using proteomic profiling. This review aims to summarize the results of studies that have used this new technology method for the early diagnosis of human cancer. Despite promising results, the use of the proteomic profiling as a diagnostic tool brought some controversies and technical problems and still requires some efforts to be standardised and validated.
Appendices
Références
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